Teaching & Learning Resources
Curated materials for biostatistics, data analysis, and neuroscience research methods
Biostatistics & R Programming — Curated Collection
A comprehensive, curated collection of books, interactive guides, and workflows for statistical analysis using R — tailored for life science researchers.
Laboratory Protocols
Optimized wetlab protocols used in our Parkinson's Disease research — freely shared for reproducibility
Western Blot Protocol — Neuro-optimized (α-Synuclein / PSMB8)
Step-by-step Western blot protocol optimized for detecting low-abundance proteins including α-synuclein aggregates and immunoproteasome subunits in human brain lysates and SH-SY5Y cell lines. Includes antibody conditions, blocking, and stripping tips.
Immunofluorescence Protocol — Frozen Brain Sections
Protocol for immunofluorescence staining of cryosectioned mouse and human post-mortem brain tissue. Optimized for co-staining dopaminergic markers (TH, DAT) with neuroinflammation markers (Iba-1, GFAP) and proteasome subunits.
RNA Extraction from Brain Tissue (TRIzol — Neuro Protocol)
Optimized RNA extraction workflow for substantia nigra and cortical brain tissue using TRIzol reagent. Includes tips for maximizing RNA integrity number (RIN ≥ 8) from archival frozen samples — critical for downstream RT-qPCR and RNA-seq.
MPTP Mouse Model — PD Induction Protocol
Detailed protocol for establishing the MPTP-induced mouse model of Parkinson's Disease. Covers acute vs. subacute dosing regimens, motor behavior assessments (rotarod, open field, pole test), and humane endpoint criteria.
Supplementary Data & Datasets
Raw and processed datasets associated with published research
Supplementary Data — eBioMedicine 2025 (PSMB8 & α-Synuclein)
Extended figures, raw western blot data, flow cytometry gating strategies, and statistical output tables for: "Pathological role of immunoproteasome PSMB8 in Parkinson's disease." eBioMedicine (2025).
Bioinformatics Code — GEO Transcriptome Analysis
R scripts for GEO database mining, differential expression analysis (DESeq2/limma), and visualization of proteasome and neuroinflammation gene signatures in substantia nigra transcriptomes from PD patients. Associated with MDS 2024 abstract.
Private Lab Repository
Restricted to invited collaborators · LPDN Laboratory, Hallym University
These materials are hosted in a private GitHub repository. Access is restricted to members of LPDN Laboratory and invited academic collaborators. Contact datneurosci@gmail.com to request access.
Lab Meeting Slides, Internal Protocols & Draft Manuscripts
Weekly lab meeting presentations, preliminary data, unpublished methodology drafts, and collaborative manuscript pre-prints.